Proposed Definition of Experimental Secondary Ischemia for Mouse Subarachnoid Hemorrhage.

Content Provider	Europe PMC
Author	van Lieshout, Jasper Hans Marbacher, Serge Muhammad, Sajjad Boogaarts, Hieronymus D. Bartels, Ronald H. M. A. Dibué, Maxine Steiger, Hans-Jakob Hänggi, Daniel Kamp, Marcel A.
Abstract	Inconsistency in outcome parameters for delayed cerebral ischemia (DCI) makes it difficult to compare results between mouse studies, in the same way inconsistency in outcome parameters in human studies has for long obstructed adequate comparison. The absence of an established definition may in part be responsible for the failed translational results. The present article proposes a standardized definition for DCI in experimental mouse models, which can be used as outcome measure in future animal studies. We used a consensus-building approach to propose a definition for “experimental secondary ischemia” (ESI) in experimental mouse subarachnoid hemorrhage that can be used as an outcome measure in preclinical studies. We propose that the outcome measure should be as follows: occurrence of focal neurological impairment or a general neurological impairment compared with a control group and that neurological impairment should occur secondarily following subarachnoid hemorrhage (SAH) induction compared with an initial assessment following SAH induction. ESI should not be used if the condition can be explained by general anesthesia or if other means of assessments sufficiently explain function impairment. If neurological impairment cannot reliably be evaluated, due to scientific setup. Verification of a significant secondary impairment of the cerebral perfusion compared with a control group is mandatory. This requires longitudinal examination in the same animal. The primary aim is that ESI should be distinguished from intervention-related ischemia or neurological deficits, in order establish a uniform definition for experimental SAH in mice that is in alignment with outcome measures in human studies.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7496000&blobtype=pdf
ISSN	18684483
Journal	Translational Stroke Research [Transl Stroke Res]
Volume Number	11
DOI	10.1007/s12975-020-00796-y
PubMed Central reference number	PMC7496000
Issue Number	5
PubMed reference number	32152960
e-ISSN	1868601X
Language	English
Publisher	Springer US
Publisher Date	2020-03-09
Publisher Place	New York
Access Restriction	Open
Rights License	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020
Subject Keyword	Experimental subarachnoid hemorrhage Delayed cerebral ischemia Outcome parameter
Content Type	Text
Resource Type	Article
Subject	Cardiology and Cardiovascular Medicine Neuroscience Neurology (clinical)