Immunotyping of tumor-infiltrating lymphocytes in triple-negative breast cancer and genetic characterization.

Content Provider	Europe PMC
Author	Gomez-Macias, Gabriela Sofia Molinar-Flores, Guillermo Lopez-Garcia, Carlos A. Santuario-Facio, Sandra Decanini-Arcaute, Horacio Valero-Elizondo, Javier Treviño-Alvarado, Victor Ortiz-Lopez, Rocio Dono, Antonio Esteban-Zubero, Eduardo Alatorre-Jimenez, Moises A. Garza, Cynthia Villarreal Peña-Curiel, Omar Cardona-Huerta, Servando
Copyright Year	2020
Abstract	Tumor-infiltrating lymphocytes (TILs) reflect the host immune response against cancer cells. Immunomodulators have been recently suggested as a novel therapeutic strategy against triple-negative breast cancer (TNBC). However, the TIL profile in TNBC has not been thoroughly investigated. In the present study, the percentage, immunophenotype and genetic profiles of TILs in pre-surgical tumor samples of patients with TNBC were evaluated prior to neoadjuvant chemotherapy (NAC). Patients diagnosed with breast cancer at Hospital San José TecSalud were consecutively and prospectively enrolled in the present study between August 2011 and August 2015. The pathological response to NAC was evaluated using the de Miller-Payne and MD Anderson Cancer Center system. TIL percentage (low, intermediate, and high) was evaluated using special hematoxylin-eosin staining on the core needle biopsies. The immunophenotype of TILs was assessed by immunohistochemistry (IHC) for CD3+, CD4+ and CD8+. In addition, the gene expression profile of CD3, CD4, CD8, CD20, CD45, forkhead box P3, interleukin 6, programmed cell death 1 and CD274 molecule was assessed in all patients. A total of 26 samples from patients with TNBC prior to NAC were included in the present study. TILs were low in 30.7%, intermediate in 38.4% and elevated in 30.7% of tumors. CD3+ and CD4+ counts were associated with the pathological response to NAC (P=0.04). Finally, an overexpression pattern of CD3, CD4, CD8, CD45 and CD20 genes was observed in patients with a partial or complete pathological response. The present results demonstrated that TILs may predict the pathological response to NAC in patients with TNBC. Furthermore, a more accurate association was identified between the high expression levels of CD3, CD4, CD8, CD45 and CD20 genes and partial and complete pathological response, compared with the association between high expression and IHC alone.
ISSN	17921074
Journal	Oncology Letters
Volume Number	20
PubMed Central reference number	PMC7471657
Issue Number	5
PubMed reference number	32934708
e-ISSN	17921082
DOI	10.3892/ol.2020.12000
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-08-20
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Gomez‑Μacias et al.
Subject Keyword	tumor-infiltrating lymphocytes breast cancer triple-negative breast cancer
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology