The utility of DLA typing for transplantation medicine in canine models.

Content Provider	Europe PMC
Author	SATO, Keita MIYAMAE, Jiro SAKAI, Manabu OKANO, Masaharu KATAKURA, Fumihiko SHIBUYA, Hisashi NAKAYAMA, Tomohiro MORITOMO, Tadaaki
Copyright Year	2020
Abstract	Transplantation medicine is used for the treatment of severe canine diseases, and the dogleukocyte antigen (DLA) is considered to be important in graft rejection. However, theutility of direct sequencing of both DLA classes I and II has not been assessedthoroughly. Eight healthy beagles with identified DLA genes were divided into two sets offour dogs, each including one donor and three recipients for skin transplantation. Thefollowing recipients were selected: one dog with a complete match, one with ahaploidentical match, and one with a complete mismatch of the DLA gene with the donor.Full-thickness skin segments were obtained from each donor and transplanted to therecipients. A mixed lymphocyte reaction (MLR) assay was performed and analyzed by flowcytometry. Skin grafts of DLA haploidentical and mismatched pairs were grossly rejectedwithin 14 days, whereas in fully matched DLA pairs, survival was as long as 21 days.Histopathological evaluation also showed moderate to severe lymphocytic infiltration andnecrosis in DLA mismatched pairs. As seen in the MLR assay, the stimulation index of DLAmismatched pairs was significantly higher than that of fully matched DLA pairs in bothsets (P<0.001). The allogeneic transplantation results suggested thatit is possible to prolong transplant engraftment by completely matching the DLA genotypebetween the donor and recipient. Additionally, the MLR assay may be used as a simplifiedin vitro method to select donors.
ISSN	09167250
Volume Number	82
PubMed Central reference number	PMC7468067
Issue Number	8
PubMed reference number	32624549
Journal	The Journal of Veterinary Medical Science [J. Vet. Med. Sci]
e-ISSN	13477439
DOI	10.1292/jvms.20-0142
Language	English
Publisher	The Japanese Society of Veterinary Science
Publisher Date	2020-07-06
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the CreativeCommons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0:https://creativecommons.org/licenses/by-nc-nd/4.0/) ©2020 The Japanese Society of Veterinary Science
Subject Keyword	cell therapy dog major histocompatibility complex skin graft
Content Type	Text
Resource Type	Article
Subject	Veterinary