Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells.

Content Provider	Europe PMC
Author	Yan, Weitao Jiang, Xia Wang, Guiqi Li, Wei Zhai, Congjie Chen, Shihao Shang, Fangjian Zhao, Zengren Yu, Weifang
Copyright Year	2020
Abstract	MicroRNA (miR)-424-5p is overexpressed in colorectal cancer (CRC); however, its role, clinical significance and underlying molecular mechanism have remained to be fully elucidated. The aim of the present study was to investigate the roles of miR-424-5p in CRC and the underlying mechanisms. It was demonstrated that miR-424-5p is overexpressed in CRC, based on bioinformatics analysis using The Cancer Genome Atlas TCGA and analysis of tissue samples from patients with CRC from The First Hospital of Hebei Medical University, and the expression of miR-424-5p was associated with the depth of invasion and Dukes' staging. In CRC cells, the oncogenic roles of miR-424-5p were also verified by Cell Counting Kit-8, wound healing and Transwell assays. To identify target genes, all transcripts were compared between miR-424-5p mimic-transfected SW480 cells and mimic control cells by transcriptome sequencing. Subsequently, the differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The DEGs were revealed to be significantly enriched in the GO terms ‘serine hydrolase activity,’ ‘serine-type peptidase activity’ and ‘serine-type endopeptidase activity’. KEGG signaling pathway analysis indicated that the DEGs were significantly enriched in ‘endocytosis’, ‘regulation of actin cytoskeleton’, ‘Wnt signaling pathway’ and ‘ubiquitin-mediated proteolysis signaling pathway’. These results suggested that miR-424-5p is a potential target in the treatment of CRC.
ISSN	17921074
Journal	Oncology Letters
Volume Number	20
PubMed Central reference number	PMC7448566
Issue Number	4
PubMed reference number	32863933
e-ISSN	17921082
DOI	10.3892/ol.2020.11982
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-08-13
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Yan et al.
Subject Keyword	microRNA-424-5p colorectal cancer proliferation migration invasion
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology