MiR-330-3p functions as a tumor suppressor that regulates glioma cell proliferation and migration by targeting CELF1.

Content Provider	Europe PMC
Author	Wang, Hongbin Liu, Guijing Li, Tao Wang, Naizhu Wu, Jingkun Zhi, Hua
Copyright Year	2020
Abstract	IntroductionGlioma is a common type of neoplasm that occurs in the central nervous system. miRNAs have been demonstrated to act as critical regulators of carcinogenesis and tumor progression in multiple cancers, but the molecular mechanism of miR-330-3p in glioma remained unclear. The purpose of the study was to explore the role of miR-330-3p in glioma cell reproduction and migration.Material and methodsThe expression levels of miR-330-3p and CELF1 in 27 glioma tissue specimens and human glioma cell lines were examined by qRT-PCR and western blot. The TargetScan database was used to predict the relationship between miR-330-3p and CELF1. Then the target relationship was verified using dual-luciferase reporter assay. The effects of miR-330-3p/CELF1 on glioma cell proliferation were evaluated by MTT and colony formation assay. Wound healing assay was employed to measure the migration ability of glioma cells.ResultsMiR-330-3p was found lowly expressed in glioma tissues and cells compared with adjacent tissues and normal astrocytes, while CELF1 expression was relatively high in the glioma tissues and cells. Dual-luciferase reporter assay confirmed that miR-330-3p could directly target CELF1. Furthermore, miR-330-3p could down-regulate the expression of CELF1, therefore suppressing glioma cell reproduction and migration.ConclusionsMiR-330-3p inhibited the propagation and migration of glioma cells by repressing CELF1 expression.
ISSN	17341922
Journal	Archives of Medical Science : AMS
Volume Number	16
PubMed Central reference number	PMC7444697
Issue Number	5
PubMed reference number	32864006
e-ISSN	18969151
DOI	10.5114/aoms.2020.95027
Language	English
Publisher	Termedia Publishing House
Publisher Date	2020-05-08
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. Copyright: © 2020 Termedia & Banach
Subject Keyword	glioma miR-330-3p CELF1 proliferation migration
Content Type	Text
Resource Type	Article
Subject	Medicine