Next generation sequencing-based analysis of mitochondrial DNA characteristics in plasma extracellular vesicles of patients with hepatocellular carcinoma.

Content Provider	Europe PMC
Author	Li, Yijie Guo, Xu Guo, Shanshan Wang, Yang Chen, Lin Liu, Yang Jia, Min An, Jiaze Tao, Kaishan Xing, Jinliang
Copyright Year	2020
Abstract	Emerging evidence has revealed that mitochondrial DNA (mtDNA) is encapsulated in plasma extracellular vesicles (EVs). However, the characteristics of mtDNA in EVs from patients with cancer remain largely unexplored, which greatly limits its clinical application. Whole genome and capture-based sequencing found that EV mtDNA covered the whole mitochondrial genome. The medium fragment size in EV mtDNA was significantly larger compared with that in cell-free mtDNA [cfmtDNA; 159 vs. 109 base pairs (bp); P<0.001]. EV DNA appeared to have a higher mtDNA copy number compared with cfDNA. Of note, patients with hepatitis had >300-bp fragments in EV mtDNA compared with patients with hepatocellular carcinoma (HCC) and healthy controls. EV mtDNA fragments >300 bp in length exhibited a significantly higher proportion of EV mtDNA fragment ends than those that were ≤300 bp in length in patients with hepatitis. The EV mtDNA copy number in patients with HCC and hepatitis were significantly lower compared with those in healthy controls. Furthermore, inconsistencies in the mtDNA heteroplasmic variant were observed among HCC tissues, plasma and EVs. In conclusion, EV mtDNA exhibited different characteristics among patients with HCC, hepatitis and healthy controls, indicating the potential value of EV mtDNA as a diagnostic biomarker that complements cfmtDNA.
ISSN	17921074
Journal	Oncology Letters
Volume Number	20
PubMed Central reference number	PMC7400774
Issue Number	3
PubMed reference number	32782600
e-ISSN	17921082
DOI	10.3892/ol.2020.11831
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-07-08
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Li et al.
Subject Keyword	extracellular vesicles mitochondrial DNA hepatocellular carcinoma liquid biopsy
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology