miR-429 inhibits osteosarcoma progression by targeting HOXA9 through suppressing Wnt/β-catenin signaling pathway.

Content Provider	Europe PMC
Author	Sun, Liangzhi Wang, Libo Luan, Suxian Jiang, Yanzhou Wang, Qiang
Copyright Year	2020
Abstract	Osteosarcoma (OS) is the most commonly diagnosed malignant cancer of bone that occurs in adolescents and children. Mounting number of studies have indicated that miRNAs are increasingly playing fundamental roles in OS development. Thus, the biological function of miR-429 in OS progression was explored. The results of RT-qPCR revealed that miR-429 was downregulated in OS tissues and OS cell lines (MG-63, U2OS, Saos-2) while homeobox A9 (HOXA9) was markedly increased. Moreover, HOXA9 was confirmed as a direct target of miR-429 by using luciferase reporter assay. It was identified that miR-429 exhibited a suppressive effect on OS progression while HOXA9 showed the oncogenic function in OS progression by using MTT and Transwell assays. More importantly, rescue assays manifested that HOXA9 can partially overturn the suppressive effect of miR-429 on OS. Overexpression of miR-429 inhibited the activation of Wnt/β-catenin signaling pathway. In conclusion, miR-429 suppressed OS progression by targeting HOXA9 through Wnt/β-catenin pathway.
ISSN	17921074
Journal	Oncology Letters
Volume Number	20
PubMed Central reference number	PMC7399823
Issue Number	3
PubMed reference number	32782562
e-ISSN	17921082
DOI	10.3892/ol.2020.11766
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-06-24
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Sun et al.
Subject Keyword	osteosarcoma miR-429 HOXA9 Wnt/β-catenin
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology