Large Scale Fabrication of Ordered Gold Nanoparticle-Epoxy Surface Nanocomposites and Their Application as Label-Free Plasmonic DNA Biosensors.

Content Provider	Europe PMC
Author	Lednický, Tomáš Bonyár, Attila
Copyright Year	2020
Abstract	A robustand scalable technology to fabricate ordered gold nanoparticle arrangementson epoxy substrates is presented. The nanoparticles are synthesizedby solid-state dewetting on nanobowled aluminum templates, which areprepared by the selective chemical etching of porous anodic alumina(PAA) grown on an aluminum sheet with controlled anodic oxidation.This flexible fabrication technology provides proper control overthe nanoparticle size, shape, and interparticle distance over a largesurface area (several cm2), which enables the fine-tuningand optimization of their plasmonic absorption spectra for LSPR andSERS applications between 535 and 625 nm. The nanoparticles are transferredto the surface of epoxy substrates, which are subsequently selectivelyetched. The resulting nanomushrooms arrangements consist of orderedepoxy nanopillars with flat, disk-shaped nanoparticles on top, andtheir bulk refractive index sensitivity is between 83 and 108 nm RIU–1. Label-free DNA detection is successfully demonstratedwith the sensors by using a 20 base pair long specific DNA sequencefrom the parasite Giardia lamblia.A red-shift of 6.6 nm in the LSPR absorbance spectrum was detectedafter the 2 h hybridization with 1 μM target DNA, and the achievableLOD was around 5 nM. The reported plasmonic sensor is one of the firstsurface AuNP/polymer nanocomposites ever reported for the successfullabel-free detection of DNA.
ISSN	19448244
Journal	ACS Applied Materials & Interfaces
Volume Number	12
PubMed Central reference number	PMC7307838
Issue Number	4
PubMed reference number	31904921
e-ISSN	19448252
DOI	10.1021/acsami.9b20907
Language	English
Publisher	AmericanChemical Society
Publisher Date	2020-01-15
Access Restriction	Open
Rights License	This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. Copyright © 2020 American Chemical Society
Subject Keyword	localized surface plasmon resonance nanoparticlelattice DNA biosensor surface nanocomposite nanobowled aluminum
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Materials Science