Mimic of the Cellular Antioxidant Defense System for a Sustainable Regeneration of Nicotinamide Adenine Dinucleotide (NAD).

Content Provider	Europe PMC
Author	Jo, Seong-Min Zhang, Kai A. I. Wurm, Frederik R. Landfester, Katharina
Copyright Year	2020
Abstract	Theprolonged use of enzymes under oxidative stress is a major challengein enabling effective enzymatic reaction pathways. Herein, we reporta biomimetic antioxidant defensive strategy capable of providing adequateprotection of enzymes against superoxide-mediated oxidation. Superoxidedismutase (SOD) and catalase (CAT) were chosen as scavengers and covalentlyencapsulated into silica nanoreactors, together with glucose dehydrogenase(GDH), which simultaneously should produce the coenzyme nicotinamideadenine dinucleotide (NADH, reduced form). By the enzymatic reactionsof SOD and CAT, the interior of silica nanoreactors becomes a “ROSsafe zone” to protect the glucose-dependent NADH productionof coencapsulated GDH. We further combined this protected NADH-producingmodule with photocatalytic nanoparticles that enable the light-triggeredoxidation of NADH back to NAD+ (oxidized form). In combination,these two modules allow interconversion between NAD+ andNADH by the addition of glucose or by light irradiation (LED lampor sunlight). This protection and regeneration strategy is a versatiletool for enzyme applications for biological reactors, catalysis, orprototypes of artificial organelles or building blocks that containsfragile biomolecules and rely on the coenzyme NAD+/NADH.
ISSN	19448244
Journal	ACS Applied Materials & Interfaces
Volume Number	12
PubMed Central reference number	PMC7303963
Issue Number	23
PubMed reference number	32383848
e-ISSN	19448252
DOI	10.1021/acsami.0c05588
Language	English
Publisher	AmericanChemical Society
Publisher Date	2020-05-26
Access Restriction	Open
Rights License	This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. Copyright © 2020 American Chemical Society
Subject Keyword	silica NADregeneration biomimetic nanoreactors cell mimics antioxidant nanoreactors
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Materials Science