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The protective effect of polyphyllin I on myocardial ischemia/reperfusion injury in rats.
| Content Provider | Europe PMC |
|---|---|
| Author | Huang, Ruizhen Shu, Jia Dai, Xiaoqin Liu, Yanru Yu, Fang Shi, Gang |
| Copyright Year | 2020 |
| Abstract | BackgroundMyocardial ischemia/reperfusion (I/R) injury has become a global public health concern. An increasing amount of evidence has shown that polyphyllin I (PPI) has anti-apoptotic and antioxidant functions. This study was performed to evaluate the cardioprotective effects of PPI in a rat model of myocardial I/R injury and the underlying mechanism.MethodsWe exposed induced a rat model of I/R injury by exposing rat hearts to left anterior descending coronary artery ligation for 30 min, followed by 24 h of reperfusion. Cardiac function was analyzed by echocardiography and HE staining. Myocardial apoptosis, inflammation, and oxidative stress were detected to analyze the PPI’s role in I/R injury.ResultsThe results showed that pretreatment with PPI improved impaired histological morphology, as shown by histopathological examination. Echocardiography analysis showed that PPI increased the levels of HR, left ventricular ejection fraction (LVEF), and left ventricular wall thickness (LVWT), accompanied by decreased left ventricular end-systolic volume (LVESV). Also, PPI decreased the expression of CK-MB, Mb, cTnI, and LDH. Specifically, PPI also changed the expression of apoptotic makers (Caspase-3, Bax, and Bcl-2), inflammatory cytokines (TNF-α, IL-6, iNOS, and IL-10) and oxidative stress markers (SOD, GSH, ROS, and MDA). Notably, western blot (WB) showed that PPI treatment inhibited the phosphorylation activity of NF-κB p65.ConclusionsThe findings showed that PPI exerted a favorable protective effect on I/R injury by inhibiting the inflammatory response and oxidative stress. It offered new drug candidates for the treatment of myocardial I/R injury. |
| ISSN | 23055839 |
| Volume Number | 8 |
| PubMed Central reference number | PMC7290651 |
| Issue Number | 10 |
| PubMed reference number | 32566581 |
| Journal | Annals of Translational Medicine [Ann Transl Med] |
| e-ISSN | 23055847 |
| DOI | 10.21037/atm-20-3371 |
| Language | English |
| Publisher | AME Publishing Company |
| Publisher Date | 2020-05-01 |
| Access Restriction | Open |
| Rights License | Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0. 2020 Annals of Translational Medicine. All rights reserved. |
| Subject Keyword | Polyphyllin I (PPI) oxidant apoptosis inflammation myocardial ischemia-reperfusion |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
