Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan.

Content Provider	Europe PMC
Author	MATSUBAYASHI, Makoto SHIBAHARA, Tomoyuki MATSUO, Tomohide HATABU, Toshimitsu YAMAGISHI, Junya SASAI, Kazumi ISOBE, Takashi
Copyright Year	2020
Abstract	There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalenceof Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37 breeding farms in 21 prefectures of Japan. Wedetected oocysts of seven species at 34 of 37 breeding farms by PCR, and we identified E. brunetti at 51.5% of farms found to be positive for Eimeria. Thedifferences in the identification of Eimeria spp. between the morphology and PCR assay methods of oocysts were pronounced for E. maxima and E.necatrix. We confirmed that molecular tools were more suitable for accurately estimating prevalence of Eimeria spp., and these findings suggest that E.brunetti could be widespread in Japan.
ISSN	09167250
Volume Number	82
PubMed Central reference number	PMC7273590
Issue Number	5
PubMed reference number	32238624
Journal	The Journal of Veterinary Medical Science [J. Vet. Med. Sci]
e-ISSN	13477439
DOI	10.1292/jvms.19-0661
Language	English
Publisher	The Japanese Society of Veterinary Science
Publisher Date	2020-04-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) ©2020 The Japanese Society of Veterinary Science
Subject Keyword	breeding farm chicken Eimeria Eimeria brunetti PCR
Content Type	Text
Resource Type	Article
Subject	Veterinary