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Quantification of mitochondrial DNA damage and copy number in circulating blood of patients with systemic sclerosis by a qPCR-based assay.
| Content Provider | Europe PMC |
|---|---|
| Author | Movassaghi, Shafieh Jafari, Sara Falahati, Kowsar Ataei, Mitra Sanati, Mohammad Hossein Jadali, Zohreh |
| Copyright Year | 2020 |
| Abstract | BackgroundAlthough not fully understood, oxidative stress has been implicated in the pathogenesis of different autoimmune diseases such as systemic sclerosis. Accumulating evidence indicates that oxidative stress can induce mitochondrial DNA (mtDNA) damage and variations in mtDNA copy number (mtDNAcn).ObjectiveThe aim of this study was to explore mtDNAcn and oxidative DNA damage byproducts in peripheral blood of patients with systemic sclerosis and healthy controls.MethodsForty six patients with systemic sclerosis and forty nine healthy subjects were studied. Quantitative real-time PCR used to measure the relative mtDNAcn and the oxidative damage (oxidized purines) of each sample.ResultsThe mean mtDNAcn was lower in patients with systemic sclerosis than in healthy controls whereas the degree of mtDNA damage was significantly higher in cases as compared to controls. Moreover, there was a negative correlation between mtDNAcn and oxidative DNA damage.Study limitationsThe lack of simultaneous analysis and quantification of DNA oxidative damage markers in serum or urine of patients with systemic sclerosis and healthy controls.ConclusionThese data suggest that alteration in mtDNAcn and increased oxidative DNA damage may be involved in the pathogenesis of systemic sclerosis. |
| ISSN | 03650596 |
| Journal | Anais Brasileiros de Dermatologia |
| Volume Number | 95 |
| PubMed Central reference number | PMC7253925 |
| Issue Number | 3 |
| PubMed reference number | 32307203 |
| e-ISSN | 18064841 |
| DOI | 10.1016/j.abd.2019.11.003 |
| Language | English |
| Publisher | Sociedade Brasileira de Dermatologia |
| Publisher Date | 2020-03-20 |
| Access Restriction | Open |
| Rights License | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). © 2020 Sociedade Brasileira de Dermatologia. Published by Elsevier España, S.L.U. |
| Subject Keyword | Autoimmunity DNA Mitochondrial Oxidative stress Real-time polymerase chain reaction Scleroderma Systemic |
| Content Type | Text |
| Resource Type | Article |
| Subject | Dermatology |
