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Energy Metabolism Analysis of Three Different Mesenchymal Stem Cell Populations of Umbilical Cord Under Normal and Pathologic Conditions.
| Content Provider | Europe PMC |
|---|---|
| Author | Russo, Eleonora Lee, Jea-Young Nguyen, Hung Corrao, Simona Anzalone, Rita La Rocca, Giampiero Borlongan, Cesar V. |
| Abstract | Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a pivotal source of therapeutically active cells for regenerative medicine due to their multipotent differentiation potential, immunomodulatory and anti-inflammatory proprieties, as well as logistical collection advantages without ethical concerns. However, it remains poorly understood whether MSCs from different compartments of the human umbilical cord are therapeutically superior than others. In this study, MSCs were isolated from Wharton’s jelly (WJ-MSCs), perivascular region (PV-MSCs) and cord lining (CL-MSCs) of hUC. These cells expressed the mesenchymal markers (CD90, CD73), stemness marker (OCT4), endothelial cell adhesion molecular marker (CD146), and the monocyte/macrophage marker (CD14) found within the MSC population implicated as a key regulator of inflammatory responses to hypoxia, was displayed by WJ-, PV-, and CL-MSCs respectively. A direct consequence of oxygen and glucose deprivation during stroke and reperfusion is impaired mitochondrial function that contributes to cellular death. Emerging findings of mitochondria transfer provide the basis for the replenishment of healthy mitochondria as a strategy for the treatment of stroke. Cell Energy Phenotype and Mito Stress tests were performed the energy metabolic profile of the three MSC populations and their mitochondrial function in both ambient and OGD cell culture conditions. PV-MSCs showed the highest mitochondrial activity. CL-MSCs were the least affected by OGD/R condition, suggesting their robust survival in ischemic environment. In this study, MSC populations in UC possess comparable metabolic capacities and good survival under normal and hypoxic conditions suggesting their potential as transplantable cells for mitochondrial-based stem cell therapy in stroke and other ischemic diseases. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7253397&blobtype=pdf |
| ISSN | 26293269 |
| Journal | Stem Cell Reviews and Reports [Stem Cell Rev Rep] |
| Volume Number | 16 |
| DOI | 10.1007/s12015-020-09967-8 |
| PubMed Central reference number | PMC7253397 |
| Issue Number | 3 |
| PubMed reference number | 32185666 |
| e-ISSN | 26293277 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2020-06-01 |
| Publisher Place | New York |
| Access Restriction | Open |
| Rights License | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020 |
| Subject Keyword | Umbilical cord mesenchymal stem cells Wharton’s Jelly Perivascular Stroke Ischemic diseases Mitochondria Bioenergetics Stem cell therapy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Cancer Research |
