Downregulation of miR‑142‑5p inhibits human aortic smooth muscle cell proliferation and migration by targeting MKL2.

Content Provider	Europe PMC
Author	Wu, Wei Shang, Yuqiang Dai, Shiling Yu, Chunjun Wang, Jie
Copyright Year	2020
Abstract	The increased proliferation and migration of vascular smooth muscle cells (VSMCs) are critical in the progression of atherosclerosis (AS). Platelet-derived growth factor type BB (PDGF-BB) may induce VSMC proliferation and migration. miR-142-5p plays a critical role in various biological processes, including tumorigenesis, angiogenesis and inflammation. However, whether miR-142-5p is involved in regulating the pathological process of arteriosclerosis remains to be elucidated. Therefore, in this study, the role of miR-142-5p in PDGF-BB-induced human aortic smooth muscle cell (HSAMC) proliferation and migration was investigated. The results revealed that the expression level of miR-142-5p was enhanced in the serum of patients with AS, while that of its target gene, myocardin-like protein 2 (MKL2) was decreased, compared with that in healthy volunteers. Moreover, there was a negative correlation between miR-142-5p and MKL2 expression in the serum of patients with AS. Furthermore, the downregulation of miR-142-5p inhibited PDGF-BB-induced HASMC proliferation and migration; however, the inhibition of HASMC proliferation and migration was reversed by co-transfection with small interfering RNA (siRNA) against MKL2 (siRNA-MKL2). In addition, transfection with miR-142-5p inhibitor significantly increased the expression levels of MKL2, and decreased those of matrix metalloproteinase (MMP)2 and 9, and these effects were reversed by transfection with siRNA-MKL2. Finally, MKL2 was proven to be a target of miR-142-5p. On the whole, the findings of the present study demonstrate that the downregulation of miR-142-5p inhibits human aortic smooth muscle cell (HSAMC) proliferation and migration possibly by targeting MKL2. Hence, miR-142-5p may prove to be a novel therapeutic target in the treatment of AS.
ISSN	17912997
Journal	Molecular Medicine Reports
PubMed Central reference number	PMC7248461
Issue Number	1
PubMed reference number	32626937
e-ISSN	17913004
DOI	10.3892/mmr.2020.11093
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-04-24
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Wu et al.
Subject Keyword	microRNA-142-5p myocardin-like protein 2 atherosclerosis proliferation and migration human aortic smooth muscle
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Biochemistry Cancer Research Molecular Medicine Oncology