The R2R3-MYB transcription factor GhMYB1a regulates flavonol and anthocyanin accumulation in Gerbera hybrida.

Content Provider	Europe PMC
Author	Zhong, Chunmei Tang, Yi Pang, Bin Li, Xukun Yang, Yuping Deng, Jing Feng, Chengyong Li, Lingfei Ren, Guiping Wang, Yaqin Peng, Jianzong Sun, Shulan Liang, Shan Wang, Xiaojing
Abstract	Anthocyanins and flavonols have vital roles in flower coloration, plant development, and defense. Because anthocyanins and flavonols share the same subcellular localization and common biosynthetic substrates, these pathways may compete for substrates. However, the mechanism regulating this potential competition remains unclear. Here, we identified GhMYB1a, an R2R3-MYB transcription factor involved in the regulation of anthocyanin and flavonol accumulation in gerbera (Gerbera hybrida). GhMYB1a shares high sequence similarity with that of other characterized regulators of flavonol biosynthesis. In addition, GhMYB1a is also phylogenetically grouped with these proteins. The overexpression of GhMYB1a in gerbera and tobacco (Nicotiana tabacum) resulted in decreased anthocyanin accumulation and increased accumulation of flavonols by upregulating the structural genes involved in flavonol biosynthesis. We further found that GhMYB1a functions as a homodimer instead of interacting with basic helix-loop-helix cofactors. These results suggest that GhMYB1a is involved in regulating the anthocyanin and flavonol metabolic pathways through precise regulation of gene expression. The functional characterization of GhMYB1a provides insight into the biosynthesis and regulation of flavonols and anthocyanins.
ISSN	26626810
Journal	Horticulture Research
Volume Number	7
PubMed Central reference number	PMC7237480
PubMed reference number	32435501
e-ISSN	20527276
DOI	10.1038/s41438-020-0296-2
Language	English
Publisher	Nature Publishing Group UK
Publisher Date	2020-05-20
Publisher Place	London
Access Restriction	Open
Rights License	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020
Subject Keyword	Plant physiology Plant morphogenesis
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Biochemistry Horticulture Biotechnology