A promising wound dressing based on alginate hydrogels containing vitamin D3 cross-linked by calcium carbonate/d-glucono-δ-lactone.

Content Provider	Europe PMC
Author	Ehterami, Arian Salehi, Majid Farzamfar, Saeed Samadian, Hadi Vaez, Ahmad Sahrapeyma, Hamed Ghorbani, Sadegh
Abstract	In the present study, we fabricated vitamin D3-loaded alginate hydrogel and assessed its wound healing capability in the animal model. The various concentrations of vitamin D3 were added to the pre-dissolved sodium alginate in deionized water and cross-linked by calcium carbonate in combination with d-glucono-δ-lactone. The microstructure, swelling behavior, weight loss, hemo- and cytocompatibility of the fabricated hydrogels were evaluated. In the last stage, the therapeutic efficacy of the prepared hydrogels was evaluated in the full-thickness dermal wound model. The scanning electron microscopy images showed that the prepared hydrogel was highly porous with the porosity of 89.2 ± 12.5% and contained the interconnected pores. Weight loss assessment showed that the prepared hydrogel is biodegradable with the weight loss percentage of about 89% in 14 days. The results showed that the prepared hydrogels were hemo- and cytocompatible. The animal study results implied that alginate hydrogel/3000 IU vitamin D3 group exhibited the highest wound closure present which was statistically significant than the control group (p < 0.05). Moreover, the histological examinations revealed that hydrogel containing 3000 IU vitamin D3 had the best performance and induced the highest re-epithelialization and granular tissue formation. All in all, this study suggests that alginate hydrogels with 3000 IU vitamin D3 can be exploited as a potential wound dressing in skin tissue engineering.
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ISSN	20939868
Volume Number	10
DOI	10.1007/s13534-020-00155-8
PubMed Central reference number	PMC7235134
Issue Number	2
PubMed reference number	32431957
Journal	Biomedical Engineering Letters [Biomed Eng Lett]
e-ISSN	2093985X
Language	English
Publisher	The Korean Society of Medical and Biological Engineering
Publisher Date	2020-03-19
Publisher Place	Korea
Access Restriction	Open
Rights License	© Korean Society of Medical and Biological Engineering 2020
Subject Keyword	Tissue engineering Alginate hydrogel Vitamin D3 Skin Wound healing
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering