Factors of Endoscopic Ultrasound-Guided Tissue Acquisition for Successful Next-Generation Sequencing in Pancreatic Ductal Adenocarcinoma.

Content Provider	Europe PMC
Author	Park, Jae Keun Lee, Ji Hyeon Noh, Dong Hyo Park, Joo Kyung Lee, Kyu Taek Lee, Jong Kyun Lee, Kwang Hyuck Jang, Kee-Taek Cho, Juhee
Copyright Year	2020
Abstract	Background/AimsRecent advances in understanding the genetics of pancreatic ductal adenocarcinoma (PDAC) have led to the potential for a personalized approach. Several studies have described the feasibility of generating genetic profiles of PDAC with next-generation sequencing (NGS) of samples obtained through endoscopic ultrasound-guided tissue acquisition (EUS-TA). The aim of this study was to find the best EUS-TA approach for successful NGS of PDAC.MethodsWe attempted to perform NGS with tissues from 190 patients with histologically proven PDAC by endoscopic ultrasound-guided fine-needle aspiration and endoscopic ultrasound-guided fine-needle biopsy at Samsung Medical Center between November 2011 and February 2015. The medical records of these patients were retrospectively reviewed for parameters including tumor factors (size, location, and T stage), EUS-TA factors (needle gauge [G], needle type, and number of needle passes) and histologic factors (cellularity and blood contamination). The sample used for NGS was part of the EUS-TA specimen that underwent cytological and histological analysis.ResultsNGS could be successfully performed in 109 patients (57.4%). In the univariate analysis, a large needle G (p=0.003) and tumor located in the body/tail (p=0.005) were associated with successful NGS. The multivariate logistic regression analysis revealed that the needle G was an independent factor of successful NGS (odds ratio, 2.19; 95% confidence interval, 1.08 to 4.47; p=0.031).ConclusionsThe needle G is an independent factor associated with successful NGS. This finding may suggest that the quantity of cells obtained from EUS-TA specimens is important for successful NGS.
ISSN	19762283
Journal	Gut and Liver
Volume Number	14
PubMed Central reference number	PMC7234878
Issue Number	3
PubMed reference number	31581388
e-ISSN	20051212
DOI	10.5009/gnl19011
Language	English
Publisher	Editorial Office of Gut and Liver
Publisher Date	2020-05-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2020 by The Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, Korean College of Helicobacter and Upper Gastrointestinal Research, Korean Association the Study of Intestinal Diseases, the Korean Association for the Study of the Liver, Korean Pancreatobiliary Association, and Korean Society of Gastrointestinal Cancer.
Subject Keyword	Next-generation sequencing Endoscopic ultrasound-guided tissue acquisition Endoscopic ultrasound guided fine-needle aspiration Endoscopic ultrasound guided fine-needle biopsy Pancreatic ductal adenocarcinoma
Content Type	Text
Resource Type	Article
Subject	Hepatology Gastroenterology