A Novel Anti-Microbial Peptide from Pseudomonas, REDLK Induced Growth Inhibition of Leishmania tarentolae Promastigote In Vitro.

Content Provider	Europe PMC
Author	Yu, Yanhui Zhao, Panpan Cao, Lili Gong, Pengtao Yuan, Shuxian Yao, Xinhua Guo, Yanbing Dong, Hang Jiang, Weina
Copyright Year	2020
Abstract	Leishmaniasis is a prevalent cause of death and animal morbidity in underdeveloped countries of endemic area. However, there is few vaccine and effective drugs. Antimicrobial peptides are involved in the innate immune response in many organisms and are being developed as novel drugs against parasitic infections. In the present study, we synthesized a 5-amino acid peptide REDLK, which mutated the C-terminus of Pseudomonas exotoxin, to identify its effect on the Leishmania tarentolae. Promastigotes were incubated with different concentration of REDLK peptide, and the viability of parasite was assessed using MTT and Trypan blue dye. Morphologic damage of Leishmania was analyzed by light and electron microscopy. Cellular apoptosis was observed using the annexin V-FITC/PI apoptosis detection kit, mitochondrial membrane potential assay kit and flow cytometry. Our results showed that Leishmania tarentolae was susceptible to REDLK in a dose-dependent manner, disrupt the surface membrane integrity and caused parasite apoptosis. In our study, we demonstrated the leishmanicidal activity of an antimicrobial peptide REDLK from Pseudomonas aeruginosa against Leishmania tarentolae in vitro and present a foundation for further research of anti-leishmanial drugs.
ISSN	00234001
Journal	The Korean Journal of Parasitology
Volume Number	58
PubMed Central reference number	PMC7231825
Issue Number	2
PubMed reference number	32418386
e-ISSN	17380006
DOI	10.3347/kjp.2020.58.2.173
Language	English
Publisher	The Korean Society for Parasitology and Tropical Medicine
Publisher Date	2020-04-30
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2020 by The Korean Society for Parasitology and Tropical Medicine
Subject Keyword	Leishmania tarentolae Pseudomonas aeruginosa leishmaniasis antimicrobial peptide REDLK
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology