Quantitative Tracking Tumor Suppression Efficiency of Human Umbilical Cord-Derived Mesenchymal Stem Cells by Bioluminescence Imaging in Mice Hepatoma Model.

Content Provider	Europe PMC
Author	Liu, Jingjing Shi, Yupeng Han, Jing Zhang, Yong Cao, Zhenghao Cheng, Jingliang
Copyright Year	2020
Abstract	Background and ObjectivesTracking of the tumor progression by MSCs-based therapy is being increasingly important in evaluating relative therapy effectively. Herein, Bioluminescence imaging (BLI) technology was used to dynamically and quantitatively track the hepatocellular carcinoma suppressive effects by human umbilical cord mesenchymal stem cells (UC-MSCs).Methods and ResultsThe stem cells present typical phenotypic characteristics and differentiation ability by morphology and flow cytometry analysis of marker expression. Then, the growth inhibition effect of conditioned medium and UC-MSC on H7402 cells was studied. It is found both the conditioned medium and UC-MSC can effectively decrease the proliferation of H7402 cells compared with the control group. Meanwhile, the relative migration of UC-MSC to H7402 is also increased through the transwell migration assay. In addition, a mice hepatoma tumor model was built by H7402 cells which can express a pLenti-6.3/DEST-CMV-luciferase 2-mKate2 gene. The effect of stem cells on growth inhibition of tumor in a mice transplantation model was dynamically monitored by bioluminescence imaging within 5 weeks. It has shown the bioluminescence signal intensity of the tumor model was significantly higher than that of the UC-MSC co-acting tumor model, indicating that the inhibition of UC-MSC on liver cancer resulted in low expression of bioluminescent signals.ConclusionsThe microenvironment of UC-MSCs can effectively inhibit the growth of liver cancer cells, and this therapeutic effect can be dynamically and quantitatively monitored in vivo by BLI. This is of great significance for the imaging research and application of stem cells in anticancer therapy.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7119203&blobtype=pdf
ISSN	20053606
Journal	International Journal of Stem Cells [Int J Stem Cells]
Volume Number	13
DOI	10.15283/ijsc19098
PubMed Central reference number	PMC7119203
Issue Number	1
PubMed reference number	31887848
e-ISSN	20055447
Language	English
Publisher	Korean Society for Stem Cell Research
Publisher Date	2020-03-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2020 by the Korean Society for Stem Cell Research
Subject Keyword	Mesenchymal stem cells Bioluminescent imaging Hepatocellular carcinoma Cancer therapy Tumor environment
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology