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Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses.

Content Provider	Europe PMC
Author	Chi, X. Sherry Li, Fenglan Tam, John S. Rappaport, Ruth Cheng, Sheau-Mei
Copyright Year	2006
Abstract	A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7112931&blobtype=pdf
ISSN	01660934
Journal	Journal of Virological Methods [J Virol Methods]
Volume Number	139
DOI	10.1016/j.jviromet.2006.08.015
PubMed Central reference number	PMC7112931
Issue Number	1
PubMed reference number	17030067
e-ISSN	18790984
Language	English
Publisher	Elsevier B.V.
Publisher Date	2006-10-09
Access Restriction	Open
Rights License	Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Copyright © 2006 Elsevier B.V. All rights reserved.
Subject Keyword	Influenza virus Respiratory syncytial virus Multiplex PCR DNA LabChip
Content Type	Text
Resource Type	Article
Subject	Virology

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