Homogeneous DNA sensing using enzyme-inhibiting DNA aptamers.

Content Provider	Europe PMC
Author	Yoshida, Wataru Sode, Koji Ikebukuro, Kazunori
Copyright Year	2006
Abstract	A novel aptameric enzyme subunit (AES) which can detect target DNAs has been developed. AES is an enzyme-inhibiting aptamer bearing a target-molecule binding site which can allosterically control enzymatic activity. The thrombin-inhibiting aptamer bearing a G-quartet structure was chosen as the enzyme-inhibiting aptamer. The stem-and-loop structure, which contains a strand complementary to the target DNA, was inserted into the G-quartet structure of the thrombin-inhibiting aptamer to disrupt the G-quartet structure through the hybridization of the target DNA with the complementary strand in the AES. The disruption of the G-quartet structure led to a decrease of the inhibitory activity of the whole aptameric complex. Using this designed aptamer, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution without bound/free separation, and the lower detection limit was 20 nM.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7092944&blobtype=pdf
ISSN	0006291X
Journal	Biochemical and Biophysical Research Communications [Biochem Biophys Res Commun]
Volume Number	348
DOI	10.1016/j.bbrc.2006.07.069
PubMed Central reference number	PMC7092944
Issue Number	1
PubMed reference number	16876760
e-ISSN	10902104
Language	English
Publisher	Elsevier Inc.
Publisher Date	2006-07-21
Access Restriction	Open
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Subject Keyword	Homogeneous DNA sensing Enzyme-inhibiting DNA aptamers Aptameric enzyme subunit SARS detection Thrombin-inhibiting aptamer
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry Biophysics