Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.

Content Provider	Europe PMC
Author	Kubota-Koketsu, Ritsuko Mizuta, Hiroyuki Oshita, Masatoshi Ideno, Shoji Yunoki, Mikihiro Kuhara, Motoki Yamamoto, Naomasa Okuno, Yoshinobu Ikuta, Kazuyoshi
Copyright Year	2009
Abstract	Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of five influenza-vaccinated volunteers, with newly developed murine–human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: one for influenza A H1N1, four for influenza A H3N2 and five for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and four (two for H3N2 and two for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the two broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7092891&blobtype=pdf
ISSN	0006291X
Journal	Biochemical and Biophysical Research Communications [Biochem Biophys Res Commun]
Volume Number	387
DOI	10.1016/j.bbrc.2009.06.151
PubMed Central reference number	PMC7092891
Issue Number	1
PubMed reference number	19580789
e-ISSN	10902104
Language	English
Publisher	Elsevier Inc.
Publisher Date	2009-07-04
Access Restriction	Open
Rights License	Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Copyright © 2009 Elsevier Inc. All rights reserved.
Subject Keyword	Influenza virus Human monoclonal antibody Virus neutralization Global epitope
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry Biophysics