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Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06.
| Content Provider | Europe PMC |
|---|---|
| Author | Ren, Xiaofeng Yin, Jiechao Ma, Dexing Li, Guangxing |
| Abstract | A Chinese isolate of avian infectious bronchitis virus (IBV) designated HH06 was isolated from the kidney tissues of a chicken flock experiencing an outbreak of nephritis. In vivo pathogenicity of the IBV isolate HH06 was determined by inoculating specific pathogen-free (SPF) chickens. The clinical signs and related gross lesions of HH06 infected chickens were similar with those of the field-infected chickens. SPF embryonated eggs were inoculated with virus suspension for serial passage and their genomic RNA was extracted. RT-PCR technique was utilized to amplify the M gene sequence encoding membrane protein of IBV. Recombinant plasmid named T-vector-M was constructed via inserting the M gene into the TA cloning vector, pMD 18-T. The sequenced M gene and its deduced amino acid (aa) sequences were compared with the published sequences of reference strains. The M gene is of 687 bp in length encoding the M protein of 228 amino acids with a predicted molecular weight of 25.4 kDa. The sequences of the M gene and M protein share 83.9–97.9% and 83.6–96.5% homologous identities, respectively, compared with 29 IBV reference strains derived from different regions or countries, which revealed that there are still significant variations between strains. Furthermore, a phylogenetic tree based on these M DNA sequences was generated, and the tree topology suggests that some Chinese IBV strains may have a common ancestor; however, HH06 is a new local IBV isolate that is responsible for the field outbreak of nephritis. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7089277&blobtype=pdf |
| ISSN | 09208569 |
| Journal | Virus Genes |
| Volume Number | 38 |
| DOI | 10.1007/s11262-008-0280-7 |
| PubMed Central reference number | PMC7089277 |
| Issue Number | 1 |
| PubMed reference number | 18792774 |
| e-ISSN | 1572994X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2008-09-16 |
| Publisher Place | New York |
| Access Restriction | Open |
| Rights License | This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. © Springer Science+Business Media, LLC 2008 |
| Subject Keyword | IBV M gene Cloning Genetic variation Phylogenetic analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Genetics Medicine Molecular Biology |
