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MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Noguchi, Satoshi Hijikata, Minako Hamano, Emi Matsushita, Ikumi Ito, Hideyuki Ohashi, Jun Nagase, Takahide Keicho, Naoto |
| Abstract | Myxovirus resistance A (MxA) is a major interferon (IFN)-inducible antiviral protein. Promoter single-nucleotide polymorphisms (SNPs) of MxA near the IFN-stimulated response element (ISRE) have been frequently associated with various viral diseases, including emerging respiratory infections. We investigated the expression profile of MxA transcripts with distinct first exons in human bronchial epithelial cells. For primary culture, the bronchial epithelium was isolated from lung tissues with different genotypes, and total RNA was subjected to real-time reverse transcription polymerase chain reaction. The previously reported MxA transcript (T1) and a recently registered transcript with a distinct 5′ first exon (T0) were identified. IFN-β and polyinosinic–polycytidylic acid induced approximately 100-fold higher expression of the T1 transcript than that of the T0 transcript, which also had a potential ISRE motif near its transcription start site. Even without inducers, the T1 transcript accounted for approximately two thirds of the total expression of MxA, levels of which were significantly associated with its promoter and exon 1 SNPs (rs17000900, rs2071430, and rs464138). Our results suggest that MxA observed in respiratory viral infections is possibly dominated by the T1 transcript and partly influenced by relevant 5′ SNPs.Electronic supplementary materialThe online version of this article (doi:10.1007/s00251-012-0663-8) contains supplementary material, which is available to authorized users. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7079882&blobtype=pdf |
| ISSN | 00937711 |
| Journal | Immunogenetics |
| Volume Number | 65 |
| DOI | 10.1007/s00251-012-0663-8 |
| PubMed Central reference number | PMC7079882 |
| Issue Number | 2 |
| PubMed reference number | 23160781 |
| e-ISSN | 14321211 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-11-18 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | Open |
| Rights License | This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. © Springer-Verlag Berlin Heidelberg 2012 |
| Subject Keyword | Myxovirus resistance A Single-nucleotide polymorphism Human bronchial epithelial cells Transcript variants |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Immunology |
