Evaluation of immunohistochemical expression of TWIST in oral epithelial dysplasia and squamous cell carcinoma.

Content Provider	Europe PMC
Author	Qahtani, Mashael S. El-Deeb, Amal M. Metwaly, Hamdy A. M.
Copyright Year	2020
Abstract	Introduction:The major transcription factor, which modulates the epithelial-mesenchymal transition in different types of cancers, is known as TWIST oncogene. It binds to the promoter of E-cadherin and suppresses its transcription. The current study aims to assess the expression of TWIST protein in oral squamous cell carcinoma (OSCC), epithelial dysplasia (ED), and normal oral mucosa to verify whether such protein is useful as a marker in oral epithelium malignant transformation.Methods:Thirty-five paraffin-embedded tissue samples of oral lesions with ED and OSCC and five samples of normal oral mucosa were immuostained with anti-TWIST antibody using the streptavidin peroxidase method.Results:TWIST expression was negative in all cases of normal oral mucosa, whereas all cases of ED and OSCC showed positive immunoreactivity to TWIST varied from weak to strong expression. In ED, there was a significant difference between severe dysplasia and the other two types (P = 0.03). TWIST expression had no significant relationship with the clinical parameters of OSSC clinical stage and grade (degree of differentiation). Only two cases of OSCC with lymph node metastasis showed strong nuclear TWIST expression. Intergroups assessment indicated a significant increase of TWIST expression in OSCC compared to ED (P = 0.000).Conclusion:A significant increase of TWIST expression in OSCC compared to ED may suggest its role in carcinogenesis, it may be a useful marker in malignant transformation of oral epithelium. Therefore, TWIST might be an important target for therapeutic approaches in patients with OSCC, which requires further investigations.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7069664&blobtype=pdf
ISSN	16583639
Journal	International Journal of Health Sciences [Int J Health Sci (Qassim)]
Volume Number	14
PubMed Central reference number	PMC7069664
Issue Number	2
PubMed reference number	32206058
e-ISSN	16587774
Language	English
Publisher	Qassim Uninversity
Publisher Date	2020-03-01
Publisher Place	Saudi Arabia
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright: © International Journal of Health Sciences
Subject Keyword	Epithelial dysplasia immunohistochemistry squamous cell carcinoma TWIST
Content Type	Text
Resource Type	Article
Subject	Medicine