Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XF^e96 Extracellular Flux Analyzer.

Content Provider	Europe PMC
Author	Preez, G. Du Fourie, H. Daneel, M. Miller, H. Höss, S. Ricci, C. Engelbrecht, G. Zouhar, M. Wepener, V.
Abstract	Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48 h (at 20 °C) to different concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XFe96 Extracellular Flux Analyzer. Dose-response curves for BAC-C16 (R2 = 0.93; P = 0.001) and Cd (R2 = 0.98; P = 0.001) were created. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 (R2 = 0.93; P = 0.0001) and Cd (R2 = 0.91; P = 0.0001). The data presented in this study show that C. elegans oxygen consumption rate can be used as a promising functional measurement of toxicity.
Journal	Scientific Reports [Sci Rep]
Volume Number	10
DOI	10.1038/s41598-020-61054-7
PubMed Central reference number	PMC7060326
Issue Number	1
PubMed reference number	32144330
e-ISSN	20452322
Language	English
Publisher	Nature Publishing Group
Publisher Date	2020-03-06
Publisher Place	London
Access Restriction	Open
Subject Keyword	Caenorhabditis elegans High-throughput screening Toxicology
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary