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miR-130a alleviates neuronal apoptosis and changes in expression of Bcl-2/Bax and caspase-3 in cerebral infarction rats through PTEN/PI3K/Akt signaling pathway.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Yinming Gu, Junquan Hu, Linlin Kong, Liang Wang, Tinggang Di, Meiqi Li, Chaosheng Gui, Shuhua |
| Copyright Year | 2020 |
| Abstract | Effect of micro ribonucleic acid (miR)-130a on neuronal apoptosis in rats with cerebral infarction (CI) was studied to explore whether phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (Akt) is involved in the regulation of neuronal apoptosis. Thirty-six Sprague-Dawley (SD) rats were randomly divided into blank control group, model group and miR-130a low-expression group. miR-130a was determined by quantitative polymerase chain reaction (qPCR), the content of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-10 was detected using the enzyme-linked immunosorbent assay (ELISA) kits, and the neuronal apoptosis level in each group was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. The neurobehavioral score was significantly lower in model group than that in blank control group (P<0.01), while it was significantly higher in miR-130a low-expression group than that in model group (P<0.01). Compared with blank control group, the model group had obviously increased content of TNF-α and IL-6 (P<0.01), decreased content of IL-10 (P<0.01), more apoptotic neurons (P<0.01), higher expression of caspase-3 (P<0.01), and obviously lower Bcl-2/Bax (P<0.01). Moreover, expression of phosphorylated (p)-PTEN, PI3K and p-Akt in brain tissues was remarkably lower in the model group than those in the blank control group (P<0.01). The expression level of miR-130a in brain tissues of CI rats is significantly increased. miR-130a promotes the release of inflammatory factors and facilitates neuronal apoptosis through suppressing the PTEN/PI3K/Akt signaling pathway. |
| ISSN | 17920981 |
| Journal | Experimental and Therapeutic Medicine |
| Volume Number | 19 |
| PubMed Central reference number | PMC7027342 |
| Issue Number | 3 |
| PubMed reference number | 32104274 |
| e-ISSN | 17921015 |
| DOI | 10.3892/etm.2020.8415 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2020-01-03 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Wang et al. |
| Subject Keyword | miR-130a cerebral infarction PTEN/PI3K/Akt signaling pathway apoptosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Cancer Research |
