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Heat stress modulates differential response in skin fibroblast cells of native cattle (Bos indicus) and riverine buffaloes (Bubalus bubalis).
| Content Provider | Europe PMC |
|---|---|
| Author | Shandilya, Umesh K. Sharma, Ankita Sodhi, Monika Mukesh, Manishi |
| Copyright Year | 2020 |
| Abstract | AbstractHeat stress in hot climates is a major cause that negatively affects dairy animals, leading to substantial economic loss. The present study was aimed to analyze the effect of heat stress on cellular and molecular levels in dermal fibroblast of cattle and buffaloes. Primary fibroblast culture was established using ear pinna tissue samples of cattle (Bos indicus) and riverine buffaloes (Bubalus Bubalis). The cells were exposed to thermal stress at 42°C for 1 h and subsequently allowed to recover and harvest at 37°C at different time points (0, 2, 4, 8, 16, and 24 h) along with control samples. Different cellular parameters viz., apoptosis, proliferation, mitochondrial membrane potential (ΔΨm), oxidative stress, along with expression pattern of heat responsive genes and miRNAs were determined. Cell viability and proliferation rate of heat-stressed fibroblasts decreased significantly (P < 0.05) albeit to a different extent in both species. The cell cytotoxicity, apoptosis, production of reactive oxygen species, and ΔΨm increased more significantly (P < 0.01) in heat stressed fibroblasts of buffalo than cattle. The pattern of heat shock proteins, inflammation/immune genes, and heat responsive miRNA showed differences in induction of their expression level in buffalo and native cattle fibroblasts. Conclusively, finding indicates that heat stress induces more profound impact on buffalo fibroblasts than native cattle fibroblasts. The differential response of cellular parameters, HSP genes, and miRNA expression could be due to better adaptive capacity of skin fibroblast of Bos indicus cattle in comparison with riverine buffaloes. |
| Page Count | 14 |
| ISSN | 01448463 |
| Volume Number | 40 |
| PubMed Central reference number | PMC7012655 |
| Issue Number | 2 |
| PubMed reference number | 31994693 |
| Journal | Bioscience Reports [Biosci. Rep] |
| e-ISSN | 15734935 |
| DOI | 10.1042/BSR20191544 |
| Language | English |
| Publisher | Portland Press Ltd. |
| Publisher Date | 2020-02-01 |
| Access Restriction | Open |
| Rights License | This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). © 2020 The Author(s). |
| Subject Keyword | Bos indicus cattle cellular resposne heat stress Riverine buffaloes skin fibroblast transcriptional respsone |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Biochemistry Biophysics |
