Apoptosis Induction by Menadione in Human Promyelocytic Leukemia HL-60 Cells.

Content Provider	Europe PMC
Author	Sa, Duck-Jin Lee, Eun-Jee Yoo, Byung-Sun
Abstract	Cell death induced by menadione (vitamin K-3,2-methyl-1,4-naphthoquinone) has been investigated in human promyelocytic leukemia HL-60 cells. Menadione was found to induce both apoptosis and necrosis in HL-60 cells. Low concentration (1~50 µM) of menadione induced apoptotic cell death, which was demonstrated by typical DNA ladder patterns on agarose gel electrophoresis and flow cytometry analysis. In contrast, a high concentration of menadione (100 µM) induced necrotic cell death, which was demonstrated by DNA smear pattern in agarose gel electrophoresis. Necrotic cell death was accompanied with a great reduction of cell viability. Menadione activated caspase-3, as evidenced by both increased protease activity and proteolytic cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP) into 85 kDa cleavage product. Caspase-3 activity was maximum at 50 µM of menadione, and very low at 100 µM of menadione. Taken together, our results showed that menadi-one induced mixed types of cell death, apoptosis at low concentrations and necrosis at high concentrations in HL-60 cells.
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ISSN	19768257
Journal	Toxicological Research [Toxicol Res]
Volume Number	25
PubMed Central reference number	PMC7006246
Issue Number	3
PubMed reference number	32038828
e-ISSN	22342753
DOI	10.5487/tr.2009.25.3.113
Language	English
Publisher	Springer Singapore
Publisher Date	2009-09-01
Publisher Place	Singapore
Access Restriction	Open
Rights License	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © Korean Society of Toxicology 2009
Subject Keyword	Menadione Apoptosis HL-60 cells Flow cytometry Caspase-3 PARP
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology