Naesohwangryeon-tang Induced Apoptosis and Autophagy in A549 Human Lung Cancer Cells.

Content Provider	Europe PMC
Author	Kim, Hong Jae Jeong, Jin-Woo Park, Cheol Choi, Yung Hyun Hong, Su Hyun
Copyright Year	2019
Abstract	ObjectivesNaesohwangryeon-tang (NHT) is a type of traditional herbal formula, however, little is known about its antitumor activity. In this study, the antitumor properties of NHT was evaluated in human lung adenocarcinoma cells.MethodsTo check the inhibitory effect of NHT, MTT assay was performed. Cell cycle analysis and detection of ROS production were conducted by flow cytometry. To evaluate the signaling pathway, Western blotting was conducted.ResultsOur results showed that the decrease of cell proliferation by NHT stimulation occurred more significantly in A549 cells than in NCI-H460 cells. In addition, NHT-induced apoptosis was associated with the activation of caspases and production of reactive oxygen species (ROS). NHT-induced apoptosis was attenuated after pretreatments with z-VAD-fmk or N-acetylcysteine, suggesting that NHT-induced apoptosis was caspase- and ROS-dependent. Interestingly, NHT treatment led to the development of autophagic vesicular organelles and upregulation of several autophagy-related genes. The pretreatment of bafilomycin A1 decreased apoptosis slightly but increased cell viability in the presence of NHT.ConclusionThese findings indicated that NHT induces both apoptosis and cell-protective autophagy in human lung cancer cells. This data suggests that NHT might be a novel herbal drug for lung cancer.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6970567&blobtype=pdf
ISSN	20936966
Journal	Journal of Pharmacopuncture [J Pharmacopuncture]
Volume Number	22
PubMed Central reference number	PMC6970567
Issue Number	4
PubMed reference number	31970025
e-ISSN	22346856
DOI	10.3831/kpi.2019.22.036
Language	English
Publisher	The Korean Pharmacopuncture Institute (KPI)
Publisher Date	2019-12-31
Access Restriction	Open
Rights License	This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2019 Korean Pharmacopuncture Institute
Subject Keyword	A549 Lung Cancer Cells Apoptosis Autophagy Caspases Naesohwangryeon-tang Reactive Oxygen Species
Content Type	Text
Resource Type	Article
Subject	Pharmacology Complementary and Alternative Medicine