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Label-free multiphoton imaging of β-amyloid plaques in Alzheimer's disease mouse models.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Shu Lin, Bingbing Lin, Guimin Sun, Caihong Lin, Ruolan Huang, Jia Tao, Jing Wang, Xingfu Wu, Yunkun Chen, Lidian Chen, Jianxin |
| Abstract | Abstract.β-Amyloid (Aβ) plaque, representing the progressive accumulation of the protein that mainly consists of Aβ, is one of the prominent pathological hallmarks of Alzheimer’s disease (AD). Label-free imaging of Aβ plaques holds the potential to be a histological examination tool for diagnosing AD. We applied label-free multiphoton microscopy to identify extracellular Aβ plaque as well as intracellular Aβ accumulation for the first time from AD mouse models. We showed that a two-photon-excited fluorescence signal is a sensitive optical marker for revealing the spatial–temporal progression and the surrounding morphological changes of Aβ deposition, which demonstrated that both extracellular and intracellular Aβ accumulations play an important role in the progression of AD. Moreover, combined with a custom-developed image-processing program, we established a rapid method to visualize different degrees of Aβ deposition by color coding. These results provide an approach for investigating pathophysiology of AD that can complement traditional biomedical procedures. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6850002&blobtype=pdf |
| Page Count | 11 |
| ISSN | 2329423X |
| Journal | Neurophotonics |
| Volume Number | 6 |
| PubMed Central reference number | PMC6850002 |
| Issue Number | 4 |
| PubMed reference number | 31737743 |
| e-ISSN | 23294248 |
| DOI | 10.1117/1.nph.6.4.045008 |
| Language | English |
| Publisher | Society of Photo-Optical Instrumentation Engineers |
| Publisher Date | 2019-10-01 |
| Access Restriction | Open |
| Rights License | © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
| Subject Keyword | Alzheimer’s disease β-amyloid plaque multiphoton microscopy two-photon-excited fluorescence second-harmonic generation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Radiology, Nuclear Medicine and Imaging Radiological and Ultrasound Technology |
