Role of metallothionein-like cadmium-binding protein (MTLCdBP) in the protective mechanism against cadmium toxicity in the testis.

Content Provider	Europe PMC
Author	OHTA, Hisayoshi QI, Yonggang OHBA, Kenichi TOYOOKA, Tatsushi WANG, Rui-Sheng
Copyright Year	2019
Abstract	The role of metallothionein (MT)-like cadmium (Cd) binding protein (MTLCdBP) inprotecting the testes against Cd toxicity was examined. In the acute Cd exposuretreatment, cadmium chloride was intraperitoneally injected at 2 mg Cd/kg to Wistar malerats. In the chronic Cd toxicity treatments, 20 mg Cd/kg/d was orally administered for 5 da week for 5, 10, and 15 wk. MT (-I,-II) and MTLCdBP were measured using ELISA and Cd-Hemmethods, respectively. Testicular tissues were immunostained with antibodies of MT-I,-II,MT-III, and MTLCdBP. Expression of HO1, OGG, iNOS, COX2, and p53 was measured by RT-PCR.Cd concentration in the testis increased dose-dependently in response to Cd exposure.MTLCdBP concentration increased markedly with increasing Cd accumulation. Significantincreases in expression of iNOS, HO1, COX2, and OGG1 were observed in the acute exposuretreatment. In the chronic oral administration group, expression of MT-I, MT-II, MT-III,iNOS, HO1, and COX2 did not change. Positive immunostaining of MTLCdBP was observed intesticular interstitial tissue. In the testis protected from Cd toxicity, MTLCdBPinduction increased significantly with increasing Cd accumulation. Our results suggestthat MTLCdBP plays an important role in protecting the testis against Cd toxicity.
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ISSN	00198366
Journal	Industrial Health [Ind Health]
Volume Number	57
DOI	10.2486/indhealth.2018-0177
PubMed Central reference number	PMC6783288
Issue Number	5
PubMed reference number	30606898
e-ISSN	18808026
Language	English
Publisher	National Institute of Occupational Safety and Health, Japan
Publisher Date	2018-12-29
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the CreativeCommons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0:https://creativecommons.org/licenses/by-nc-nd/4.0/) ©2019 National Institute of Occupational Safety andHealth
Subject Keyword	Cadmium Testicular toxicity Metallothionein (MT) metallothionein-like cadmium-binding protein (MTLCdBP) immunostaining Hemorrhagic inflammation Gene expression
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health