Recombinant mussel protein Pvfp-5β: A potential tissue bioadhesive.

Content Provider	Europe PMC
Author	Santonocito, Radha Venturella, Francesca Dal Piaz, Fabrizio Morando, Maria Agnese Provenzano, Alessia Rao, Estella Costa, Maria Assunta Bulone, Donatella San Biagio, Pier Luigi Giacomazza, Daniela Sicorello, Alessandro Alfano, Caterina Passantino, Rosa Pastore, Annalisa
Copyright Year	2019
Abstract	During their lifecycle, many marine organisms rely on natural adhesives to attach to wet surfaces for movement and self-defense in aqueous tidal environments. Adhesive proteins from mussels are biocompatible and elicit only minimal immune responses in humans. Therefore these proteins have received increased attention for their potential applications in medicine, biomaterials, and biotechnology. The Asian green mussel Perna viridis secretes several byssal plaque proteins, molecules that help anchoring the mussel to surfaces. Among these proteins, protein-5β (Pvfp-5β) initiates interactions with the substrate, displacing interfacial water molecules before binding to the surface. Here, we established the first recombinant expression in Escherichia coli of Pvfp-5β. We characterized recombinant Pvfp-5β, finding that despite displaying a CD spectrum consistent with features of a random coil, the protein is correctly folded as indicated by MS and NMR analyses. Pvfp-5β folds as a β-sheet–rich protein as expected for an epidermal growth factor-like module. We examined the effects of Pvfp-5β on cell viability and adhesion capacity in NIH-3T3 and HeLa cell lines, revealing that Pvfp-5β has no cytotoxic effects at the protein concentrations used and provides good cell-adhesion strength on both glass and plastic plates. Our findings suggest that the adhesive properties of recombinant Pvfp-5β make it an efficient surface-coating material, potentially suitable for biomedical applications including regeneration of damaged tissues.
ISSN	00219258
Volume Number	294
PubMed Central reference number	PMC6709630
Issue Number	34
PubMed reference number	31292195
Journal	The Journal of Biological Chemistry [J. Biol. Chem]
e-ISSN	1083351X
DOI	10.1074/jbc.RA119.009531
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2019-07-10
Publisher Place	U.S.A.
Access Restriction	Open
Rights License	Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license. © 2019 Santonocito et al.
Subject Keyword	adhesion biomaterials biophysics epidermal growth factor (EGF) structural biology adhesion proteins EGF-like motifs Marine proteins
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry