Conformational change within the extracellular domain of B cell receptor in B cell activation upon antigen binding.

Content Provider	Europe PMC
Author	Shen, Zhixun Liu, Sichen Li, Xinxin Wan, Zhengpeng Mao, Youxiang Chen, Chunlai Liu, Wanli
Editor	Chakraborty, Arup K Dustin, Michael L
Copyright Year	2019
Abstract	B lymphocytes use B cell receptors (BCRs) to recognize antigens. It is still not clear how BCR transduces antigen-specific physical signals upon binding across cell membrane for the conversion to chemical signals, triggering downstream signaling cascades. It is hypothesized that through a series of conformational changes within BCR, antigen engagement in the extracellular domain of BCR is transduced to its intracellular domain. By combining site-specific labeling methodology and FRET-based assay, we monitored conformational changes in the extracellular domains within BCR upon antigen engagement. Conformational changes within heavy chain of membrane-bound immunoglobulin (mIg), as well as conformational changes in the spatial relationship between mIg and Igβ were observed. These conformational changes were correlated with the strength of BCR activation and were distinct in IgM- and IgG-BCR. These findings provide molecular mechanisms to explain the fundamental aspects of BCR activation and a framework to investigate ligand-induced molecular events in immune receptors.
Journal	eLife
Volume Number	8
PubMed Central reference number	PMC6620044
PubMed reference number	31290744
e-ISSN	2050084X
DOI	10.7554/elife.42271
Language	English
Publisher	eLife Sciences Publications, Ltd
Publisher Date	2019-07-10
Access Restriction	Open
Rights License	This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. © 2019, Shen et al
Subject Keyword	B cell receptor BCR activation conformational change FRET Human
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Neuroscience Medicine Biochemistry, Genetics and Molecular Biology