NDLI: A novel method for rapid detection of a Helicobacter pylori infection using a γ-glutamyltranspeptidase-activatable fluorescent probe.

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A novel method for rapid detection of a Helicobacter pylori infection using a γ-glutamyltranspeptidase-activatable fluorescent probe.

Content Provider	Europe PMC
Author	Akashi, Taro Isomoto, Hajime Matsushima, Kayoko Kamiya, Mako Kanda, Tsutomu Nakano, Masayuki Onoyama, Takumi Fujii, Masashi Akada, Junko Akazawa, Yuko Ohnita, Ken Takeshima, Fuminao Nakao, Kazuhiko Urano, Yasuteru
Abstract	A γ-glutamyl hydroxymethyl rhodamine green probe (gGlu-HMRG) reacts with γ-glutamyltranspeptidase (GGT) and immediately produces fluorescence, is clinically applied for real-time cancers' visualization. Since Helicobacter pylori produces GGT, this study aimed to investigate whether gGlu-HMRG can be used to detect H. pylori infections. A wild-type H. pylori strain and the ggt gene-disrupted mutant were cultured and treated with gGlu-HMRG. This fluorescent probe assay was used to quantify GGT activity of H. pylori ex vivo using gastric biopsy specimens. The H. pylori diagnostic capabilities of the assay were determined from altered fluorescence intensity (FI) values at 5 min (FIV-5) and 15 minutes (FIV-15). Distinct fluorescence was identified in wild H. pylori strain, using gGlu-HMRG, whereas no fluorescence was observed in ggt gene-disrupted mutant strain. On ex vivo imaging of gGlu-HMRG, fluorescence intensity increased markedly with time in H. pylori-positive specimens; however, the H. pylori-negative specimens displayed a slight increase in FI. FIV-5 and FIV-15 differed significantly between H. pylori-positive and -negative specimens. FIV-15 differed significantly between H. pylori-positive and -eradicated group. This assay sensitivity and specificity were 75.0% and 83.3% in the antrum and 82.6% and 89.5% in the stomach body. GGT-activatable fluorescence probe is applicable for rapid diagnosis of H. pylori.
Journal	Scientific Reports [Sci Rep]
Volume Number	9
DOI	10.1038/s41598-019-45768-x
PubMed Central reference number	PMC6603024
Issue Number	1
PubMed reference number	31263136
e-ISSN	20452322
Language	English
Publisher	Nature Publishing Group
Publisher Date	2019-07-01
Publisher Place	London
Access Restriction	Open
Subject Keyword	Fluorescence imaging Gastritis
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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