NDLI: Phenotypic and molecular cytogenetic variability in calendula (Calendula officinalis L.) cultivars and mutant lines obtained via chemical mutagenesis.

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Phenotypic and molecular cytogenetic variability in calendula (Calendula officinalis L.) cultivars and mutant lines obtained via chemical mutagenesis.

Content Provider	Europe PMC
Author	Samatadze, Tatiana E. Zoshchuk, Svyatoslav A. Hazieva, Firdaus M. Yurkevich, Olga Yu Svistunova, Natalya Yu Morozov, Alexander I. Amosova, Alexandra V. Muravenko, Olga V.
Abstract	The morphological, meiotic and chromosomal variability were studied in two cultivars of Calendula officinalis L. and their mutant lines obtained though chemical mutagenesis using diethyl sulphate (DES) (0.04%, 0.08%) and dimethyl sulphate (DMS) (0.025%, 0.05%). The studied cultivars displayed different sensitivity to DMS and DES mutagens. More M1 plants with morphological changes were observed in C. officinalis cv. 'Zolotoe more' than in cv. 'Rajskij sad'. DMS and DES at low concentrations had positive effects on main agro-metrical traits in both cultivars including plant height, inflorescence diameter and number of inflorescences per plant. Dose-dependent increase in number of various meiotic abnormalities was revealed in both mutant lines. Comparative karyotype analysis and FISH-based visualization of 45S and 5S rDNA indicated a high level of karyotype stability in M1 and M2 plants. Seed treatments with DMS and DES at certain concentrations resulted in higher yields of inflorescences in M1 plants compared to the control. In M2 generation, dose-dependent reduction in the yields of inflorescences was observed. Our findings demonstrate that DMS and DES at low concentrations have great potential in calendula mutation breeding.
Journal	Scientific Reports [Sci Rep]
Volume Number	9
DOI	10.1038/s41598-019-45738-3
PubMed Central reference number	PMC6591508
Issue Number	1
PubMed reference number	31235779
e-ISSN	20452322
Language	English
Publisher	Nature Publishing Group
Publisher Date	2019-06-24
Publisher Place	London
Access Restriction	Open
Subject Keyword	Disease prevention Agricultural genetics
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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