CD3 and CD20 co-expression in a case of canine peripheral T-cell lymphoma with prominent cardiac and peripheral nerve involvement.

Content Provider	Europe PMC
Author	Nakagun, Shotaro Horiuchi, Noriyuki Watanabe, Kenichi Matsumoto, Kotaro Tagawa, Michihito Shimbo, Genya Kobayashi, Yoshiyasu
Copyright Year	2018
Abstract	An 8-y 9-mo-old male Pug dog was presented because of anorexia, hindlimb ataxia, vomiting, and progressive weight loss. Clinical examinations revealed atrophic hindlimb muscles with decreased postural reaction, enlargement of the cardiac silhouette and megaesophagus on radiograph, and reduced cardiac contractility on ultrasonography. The dog died 10 d after the initial examination, and an autopsy was performed. Grossly, the heart was enlarged, with multifocal-to-coalescing extensive plaque-like areas of discoloration on the epicardial surface. On cross-section, the ventricles were moderately dilated, and discoloration extended into the myocardium. Peripheral lymph nodes were of normal size. Histologically, atypical lymphoid cells replaced the myocardium and also extensively infiltrated peripheral nerve bundles in various organs. With immunohistochemistry, the neoplastic cells exhibited strong immunoreactivity for CD3 and CD20, and were negative for CD8, granzyme B, CD79α, and Pax5. Double-label immunofluorescence confirmed co-expression of CD3 and CD20 by the neoplastic cells. Molecular clonality analysis presented a clonal T-cell receptor gamma gene rearrangement. The case was diagnosed as a CD3+/CD20+ peripheral T-cell lymphoma with prominent cardiac and peripheral nerve involvement, indicating neurolymphomatosis.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6505798&blobtype=pdf
ISSN	10406387
Volume Number	30
DOI	10.1177/1040638718794765
PubMed Central reference number	PMC6505798
Issue Number	5
PubMed reference number	30129396
Journal	Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest]
e-ISSN	19434936
Language	English
Publisher	SAGE Publications
Publisher Date	2018-08-21
Publisher Place	Sage CA: Los Angeles, CA
Access Restriction	Open
Rights License	© 2018 The Author(s)
Subject Keyword	CD3 CD20 dogs heart neurolymphomatosis peripheral T-cell lymphoma
Content Type	Text
Resource Type	Article
Subject	Veterinary