Protein Adductomics: Methodologies for Untargeted Screening of Adducts to Serum Albumin and Hemoglobin in Human Blood Samples.

Content Provider	Europe PMC
Author	Carlsson, Henrik Rappaport, Stephen M. Törnqvist, Margareta
Copyright Year	2019
Abstract	The reaction products of electrophiles in vivo can be measured as adducts to the abundant proteins, hemoglobin (Hb), and human serum albumin (HSA), in human blood samples. During the last decade, methods for untargeted screening of such adducts, called “adductomics”, have used liquid chromatography-mass spectrometry to detect large numbers of previously unknown Hb and HSA adducts. This review presents methodologies that were developed and used in our laboratories for Hb and HSA adductomics, respectively. We discuss critical aspects regarding choice of target protein, sample preparation, mass spectrometry, data evaluation, and strategies for identification of detected unknown adducts. With this review we give an overview of these two methodologies used for protein adductomics and the precursor electrophiles that have been elucidated from the adducts.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6473736&blobtype=pdf
Volume Number	8
DOI	10.3390/ht8010006
PubMed Central reference number	PMC6473736
Issue Number	1
PubMed reference number	30857166
Journal	High-Throughput
e-ISSN	25715135
Language	English
Publisher	MDPI
Publisher Date	2019-03-08
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). © 2019 by the authors.
Subject Keyword	proteins protein adducts electrophiles adductomics mass spectrometry hemoglobin human serum albumin
Content Type	Text
Resource Type	Article
Subject	Biochemistry Bioengineering Biotechnology Biomedical Engineering