Conditioned Medium Enhances Osteogenic Differentiation of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells.

Content Provider	Europe PMC
Author	Zhong, Siying He, Xufeng Li, Yuexia Lou, Xiangxin
Abstract	Background: Recent studies have shown that induced pluripotent stem cells (iPSCs) could be differentiated into mesenchymal stem cells (MSCs) with notable advantages over iPSCs per se. In order to promote the application of iPSC-MSCs for osteoregenerative medicine, the present study aimed to assess the ability of murine iPSC-MSCs to differentiate into osteoblast phenotype. Methods: Osteogenic differentiation medium, blending mouse osteoblast-conditioned medium (CM) with basic medium (BM) at ratio 3:7, 5:5 and 7:3, were administered to iPSC-MSCs, respectively. After 14 days, differentiation was evaluated by lineage-specific morphology, histological stain, quantitative reverse transcription-polymerase chain reaction and immunostaining. Results: The osteogenesis-related genes, alp, runx2, col1 and ocn expressions suggest that culture medium consisting of CM:BM at the ratio of 3:7 enhanced the osteogenic differentiation more than other concentrations that were tested. In addition, the alkaline phosphatase activity and osteogenic marker Runx2 expression demonstrate that the combination of CM and BM significantly enhanced the osteogenic differentiation of iPSC-MSCs. Conclusion: In summary, this study has shown that osteoblast-derived CM can dramatically enhance osteogenic differentiation of iPSC-MSCs toward osteoblasts. Results from this work will contribute to optimize the osteogenic induction conditions of iPSC-MSCs and will assist in the potential application of iPSC-MSCs for bone tissue engineering.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6439034&blobtype=pdf
ISSN	17382696
Volume Number	16
DOI	10.1007/s13770-018-0173-3
PubMed Central reference number	PMC6439034
Issue Number	2
PubMed reference number	30989041
Journal	Tissue Engineering and Regenerative Medicine [Tissue Eng Regen Med]
e-ISSN	22125469
Language	English
Publisher	Springer Singapore
Publisher Date	2019-01-29
Publisher Place	Singapore
Access Restriction	Open
Rights License	© The Korean Tissue Engineering and Regenerative Medicine Society and Springer Nature B.V. 2019
Subject Keyword	Induced pluripotent stem cells Mesenchymal stem cells Osteoblasts Conditioned medium Osteogenic differentiation
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering