Effect of fecal microbiota transplantation on experimental colitis in mice.

Content Provider	Europe PMC
Author	Zhou, Julan Zhou, Zhongyin Ji, Panpan Ma, Min Guo, Jinkun Jiang, Shujuan
Copyright Year	2019
Abstract	The aim of the present study was to investigate the effect of fecal microbiota transplantation (FMT) on the acute inflammatory response in a murine model of dextran sulfate sodium (DSS)-induced colitis, and to delineate the putative underlying mechanism(s). Mice were divided into four groups, namely the normal control, DSS, 5-aminosalicylic acid (5-ASA) and FMT group. Mice in the DSS, 5-ASA and FMT groups were orally administered 3% DSS (w/v) solution for 7 days to induce colitis. On days 1, 3, 5 and 7, mice in the DSS, 5-ASA and FMT groups were respectively administered 0.5% carboxymethylcellulose sodium, 5-ASA suspension and fecal suspension by enema. The disease activity index of each mouse was calculated on a daily basis. All mice were sacrificed on day 8, and the length of their colons was measured. Myeloperoxidase (MPO) activity, and the levels of tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-10 in the colon tissues of each group were also measured. Compared with that in the DSS group, FMT ameliorated the severity of inflammation due to ulcerative colitis in mice, which was accompanied by a significantly decreased MPO activity, reduced levels of TNF-α and IL-1β, and an increased level of IL-10 in colon tissue (all P<0.05). Taken together, these results demonstrated that FMT exerted a therapeutic effect on experimental colitis in mice, and the associated mechanism is likely to involve the remodeling of the intestinal flora and regulation of intestinal T-cell immunity homeostasis.
ISSN	17920981
Journal	Experimental and Therapeutic Medicine
Volume Number	17
PubMed Central reference number	PMC6425147
Issue Number	4
PubMed reference number	30906449
e-ISSN	17921015
DOI	10.3892/etm.2019.7263
Language	English
Publisher	D.A. Spandidos
Publisher Date	2019-02-13
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Zhou et al.
Subject Keyword	fecal microbiota transplantation intestinal symbiotic flora ulcerative colitis immunity homeostasis dysbacteriosis
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Cancer Research