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miR-127 suppresses gastric cancer cell migration and invasion via targeting Wnt7a.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Linlin Wang, Xufei Jiang, Xuefeng |
| Copyright Year | 2019 |
| Abstract | Gastric cancer (GC) is a malignant tumor originating from the mucosal epithelium of the stomach. Patients suffering from this disease may have occurrence of residual GC due to delay in diagnosis and treatment. In addition, abnormal expression of microRNAs (miRNAs) is involved in GC progression. Therefore, we examined the underlying mechanism of miR-127 in GC. The expression of miR-127 and Wnt7a was examined in GC using RT-qPCR and western blot analysis. A Transwell assay was used to assess the ability of GC cell migration and invasion. Luciferase reporter assay was used to verify the specific target of miR-127 in GC. The results showed miR-127 expression was lower in GC than normal samples, while Wnt7a expression was detected at a higher level in GC than normal samples. The association between miR-127 and Wnt7a expression was negatively correlated in GC tissues. miR-127 mimic in the two GC cell lines markedly curbed cell migration and invasion, while inhibition of miR-127 showed the opposite effect. In addition, Wnt7a siRNA significantly inhibited GC cell migration and invasion and Wnt7a was verified as a specific target of miR-127 in GC cells. Wnt7a reversed the ability of GC cell migration and invasion regulated by miR-127. In conclusion, miR-127 could curb GC cell migration and invasion by upregulating Wnt7a, indicating its potential application in GC diagnosis and therapy. |
| ISSN | 17921074 |
| Journal | Oncology Letters |
| Volume Number | 17 |
| PubMed Central reference number | PMC6396225 |
| Issue Number | 3 |
| PubMed reference number | 30867752 |
| e-ISSN | 17921082 |
| DOI | 10.3892/ol.2019.9955 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2019-01-21 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Wang et al. |
| Subject Keyword | miR-127 migration invasion gastric cancer Wnt7a |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
