NDLI: Thiocoraline mediates drug resistance in MCF-7 cells via PI3K/Akt/BCRP signaling pathway.

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Thiocoraline mediates drug resistance in MCF-7 cells via PI3K/Akt/BCRP signaling pathway.

Content Provider	Europe PMC
Author	Jin, Jin Zhao, Yujia Guo, Wan Wang, Bingrong Wang, Yigang Liu, Xinyuan Xu, Chuanlian
Abstract	Thiocoraline, a depsipeptide bisintercalator with potent antitumor activity, was first isolated from marine actinomycete Micromonospora marina. It possesses an intense toxicity to MCF-7 cells at nanomolar concentrations in a dose-dependent manner evaluated by MTT assay and crystal violet staining. We established a human breast thiocoraline-resistant cancer subline of MCF-7/thiocoraline (MCF-7/T) to investigate the expression variation of breast cancer resistance proteins (BCRP) and its subsequent influence on drug resistance. Colony-forming assay showed that the MCF-7 cells proliferated faster than the MCF-7/T cells in vitro. Western blot analysis demonstrated that thiocoraline increased the phosphorylation of Akt. Additionally, the sensitivity of tumor cells to thiocoraline was reduced with a concurrent rise in phosphorylation level of Akt and of BCRP expression.These studies indicated that thiocoraline probably mediated the drug resistance via PI3K/Akt/BCRP signaling pathway. MK-2206 dihydrochloride, a selective phosphorylation inhibitor of Akt, significantly decreased MCF-7 cell viability under exposure to thiocoraline compared to the control. However, it was not obviously able to decrease MCF-7/T cell viability when cells were exposed to thiocoraline. Electronic supplementary material The online version of this article (10.1007/s10616-019-00301-w) contains supplementary material, which is available to authorized users.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6368516&blobtype=pdf
ISSN	09209069
Volume Number	71
DOI	10.1007/s10616-019-00301-w
PubMed Central reference number	PMC6368516
Issue Number	1
PubMed reference number	30689149
Journal	Cytotechnology
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2019-01-28
Publisher Place	Dordrecht
Access Restriction	Open
Rights License	© Springer Nature B.V. 2019
Subject Keyword	Thiocoraline MCF-7 Multidrug resistance BCRP PI3K/Akt/BCRP signaling pathway MK-2206 dihydrochloride
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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