Identification, expression, and phylogenetic analyses of terpenoid biosynthesis-related genes in secondary xylem of loblolly pine (Pinus taeda L.) based on transcriptome analyses.

Content Provider	Europe PMC
Author	Mao, Jipeng He, Zidi Hao, Jing Liu, Tianyi Chen, Jiehu Huang, Shaowei
Editor	Silva, Pedro
Copyright Year	2019
Abstract	Loblolly pine (Pinus taeda L.) is one of the most important species for oleoresin (a mixture of terpenoids) in South China. The high oleoresin content of loblolly pine is associated with resistance to bark beetles and other economic benefits. In this study, we conducted transcriptome analyses of loblolly pine secondary xylem to gain insight into the genes involved in terpenoid biosynthesis. A total of 372 unigenes were identified as being critical for oleoresin production, including genes for ATP-binding cassette (ABC) transporters, the cytochrome P450 (CYP) protein family, and terpenoid backbone biosynthesis enzymes. Six key genes involved in terpenoid biosynthetic pathways were selected for multiple sequence alignment, conserved motif prediction, and phylogenetic and expression profile analyses. The protein sequences of all six genes exhibited a higher degree of sequence conservation, and upstream genes were relatively more conserved than downstream genes in terpenoid biosynthetic pathways. The N-terminal regions of these sequences were less conserved than the C-terminal ends, as the N-terminals were quite diverse in both length and composition. The phylogenetic analyses revealed that most genes originated from gene duplication after species divergence, and partial genes exhibited incomplete lineage sorting. In addition, the expression profile analyses showed that all six genes exhibited high expression levels during the high-oleoresin-yielding phase.
Journal	Peerj
Volume Number	7
PubMed Central reference number	PMC6360084
PubMed reference number	30723613
e-ISSN	21678359
DOI	10.7717/peerj.6124
Language	English
Publisher	PeerJ Inc.
Publisher Date	2019-01-31
Publisher Place	San Diego, USA
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. ©2019 Mao et al.
Subject Keyword	Loblolly pine (Pinus taeda L.) Transcriptome analysis Terpenoid biosynthesis Phylogenetic analysis Conserved motifs
Content Type	Text
Resource Type	Article
Subject	Neuroscience Medicine Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology