NDLI: Messenger RNA Delivery for Tissue Engineering and Regenerative Medicine Applications.

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Messenger RNA Delivery for Tissue Engineering and Regenerative Medicine Applications.

Content Provider	Europe PMC
Author	Patel, Siddharth Athirasala, Avathamsa Menezes, Paula P. Ashwanikumar, N. Zou, Ting Sahay, Gaurav Bertassoni, Luiz E.
Copyright Year	2019
Abstract	The ability to control cellular processes and precisely direct cellular reprogramming has revolutionized regenerative medicine. Recent advances in in vitro transcribed (IVT) mRNA technology with chemical modifications have led to development of methods that control spatiotemporal gene expression. Additionally, there is a current thrust toward the development of safe, integration-free approaches to gene therapy for translational purposes. In this review, we describe strategies of synthetic IVT mRNA modifications and nonviral technologies for intracellular delivery. We provide insights into the current tissue engineering approaches that use a hydrogel scaffold with genetic material. Furthermore, we discuss the transformative potential of novel mRNA formulations that when embedded in hydrogels can trigger controlled genetic manipulation to regenerate tissues and organs in vitro and in vivo. The role of mRNA delivery in vascularization, cytoprotection, and Cas9-mediated xenotransplantation is additionally highlighted. Harmonizing mRNA delivery vehicle interactions with polymeric scaffolds can be used to present genetic cues that lead to precise command over cellular reprogramming, differentiation, and secretome activity of stem cells–an ultimate goal for tissue engineering.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6352544&blobtype=pdf
Page Count	22
ISSN	19373341
Volume Number	25
DOI	10.1089/ten.tea.2017.0444
PubMed Central reference number	PMC6352544
Issue Number	1-2
PubMed reference number	29661055
Journal	Tissue Engineering. Part A [Tissue Eng Part A]
e-ISSN	1937335X
Language	English
Publisher	Mary Ann Liebert, Inc., publishers
Publisher Date	2018-06-07
Publisher Place	USA
Access Restriction	Open
Rights License	Copyright 2019, Mary Ann Liebert, Inc., publishers
Subject Keyword	mRNA delivery gene therapy cell reprogramming tissue engineering lipid nanoparticles hydrogels
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biochemistry Bioengineering Biomedical Engineering

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