Loading...
Please wait, while we are loading the content...
Echinacoside protects retinal ganglion cells from ischemia/reperfusion-induced injury in the rat retina.
| Content Provider | Europe PMC |
|---|---|
| Author | Li, Lin Wang, YeFei Qin, XiuHong Zhang, Jing Zhang, ZhenZhen |
| Copyright Year | 2018 |
| Abstract | ObjectiveTo investigate whether echinacoside (ECH) protects the retina against ischemia/reperfusion (I/R) injury and the underlying mechanisms.MethodsAdult male Wistar rats were randomly divided into four groups: sham, sham plus ECH, I/R plus vehicle, and I/R plus ECH. Before the retinal I/R injury produced by high intraocular pressure (HOP), ECH was administered (20 mg/kg daily) for 7 days. The level of retinal cell damage was evaluated using Fluoro-Gold (FG) retrograde labeling and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) analysis 7 days after I/R. Optic nerve histology was analyzed with transmission electron microscopy. Levels of retinal malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were determined. The expression of apoptosis-associated factors (Apaf-1, Parp, and Bad) were analyzed with western blotting and quantitative real-time PCR (qPCR). The production of proinflammatory cytokines (tumor necrosis factor-α [TNFα], interleukin-1 beta [IL-1β], and IL-6) was analyzed with enzyme-linked immunosorbent assay (ELISA) 7 days after the I/R injury as well.ResultsThe administration of ECH not only preserved retinal morphology but also attenuated retinal inflammation and apoptosis at 7 days after the I/R injury and decreased I/R-induced oxidative stress in the retina statistically significantly.ConclusionsECH protected against I/R-induced retinal injury, via activation of antioxidant enzymes and suppression of inflammation. Therefore, ECH could be a potential therapeutic candidate for the treatment and management of I/R retinal diseases. |
| Journal | Molecular Vision |
| Volume Number | 24 |
| PubMed Central reference number | PMC6279312 |
| PubMed reference number | 30581281 |
| e-ISSN | 10900535 |
| Language | English |
| Publisher | Molecular Vision |
| Publisher Date | 2018-11-25 |
| Access Restriction | Open |
| Rights License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. Copyright © 2018 Molecular Vision. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ophthalmology |
