Karyotype and chromosomal characteristics of rDNA of Cobitisstrumicae Karaman, 1955 (Teleostei, Cobitidae) from Lake Volvi, Greece.

Content Provider	Europe PMC
Author	Hnátková, Eva Triantaphyllidis, Costas Ozouf-Costaz, Catherine Lukáš Choleva Majtánová, Zuzana Bohlen, Joerg Ráb, Petr
Abstract	AbstractThe karyotype of Greek cobitid fish Cobitisstrumicae Karaman, 1955, from Lake Volvi, Greece, a representative of one of its two major intraspecific phylogenetic lineages, was analysed by means of sequential Giemsa-staining, C-banding, silver-staining, CMA3 fluorescence banding and also by in situ hybridization (FISH) with rDNA probe. The diploid chromosome number was 2n = 50, karyotype composed of 10 pairs of metacentric to submetacentric and 15 pairs of subtelocentric to acrocentric chromosomes. The nucleolus organizer regions (NORs) as revealed by Ag- and CMA3 staining and FISH were situated in the telomeric region of the fourth submetacentric chromosome pair. The chromosomes contained very low content of C-positive heterochromatin. No heteromorphic sex chromosomes were detected. This first karyotype report for any species of lineage Bicanestrinia Băcescu, 1962 shows a simple karyotype dominated by acrocentric chromosomes and possessing single NOR-bearing chromosome pair. Cytotaxonomic implications of this finding for the taxonomy of the genus Cobitis Linnaeus, 1758 are further discussed.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6251958&blobtype=pdf
ISSN	19930771
Journal	Comparative Cytogenetics [Comp Cytogenet]
Volume Number	12
PubMed Central reference number	PMC6251958
Issue Number	4
PubMed reference number	30498563
e-ISSN	1993078X
DOI	10.3897/compcytogen.v12i4.28068
Language	English
Publisher	Pensoft Publishers
Publisher Date	2018-11-16
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Eva Hnátková, Costas Triantaphyllidis, Catherine Ozouf-Costaz, Lukáš Choleva, Zuzana Majtánová, Joerg Bohlen, Petr Ráb
Subject Keyword	chromosome banding NOR phenotype FISH rDNA cytotaxonomy of Cobitis loaches
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Animal Science and Zoology Biotechnology Insect Science