High-Throughput Screening of Biodiversity for Antibiotic Discovery.

Content Provider	Europe PMC
Author	Terekhov, S. S. Osterman, I. A. Smirnov, I. V.
Copyright Year	2018
Abstract	The increasing number of infections caused by antibiotic-resistant strains ofpathogens challenges modern technologies of drug discovery. Combinatorialchemistry approaches are based on chemical libraries. They enable the creationof high-affinity low-molecular-weight ligands of the therapeuticallysignificant molecular targets of human cells, thus opening an avenue toward adirected design of highly effective therapeutic agents. Nevertheless, theseapproaches face insurmountable difficulties in antibiotic discovery. Naturalcompounds that have evolved for such important characteristics as broadspecificity and efficiency are a good alternative to chemical libraries.However, unrestricted use of natural antibiotics and their analogues leads toavalanche-like spread of resistance among bacteria. The search for new naturalantibiotics, in its turn, is extremely complicated nowadays by the problem ofantibiotic rediscovery. This calls for the application of alternativehigh-throughput platforms for antibiotic activity screening, cultivation of“unculturable” microorganisms, exploration of novel antibioticbiosynthetic gene clusters, as well as their activation and heterologousexpression. Microfluidic technologies for the screening of antibiotic activityat the level of single cells are, therefore, of great interest, since theyenable the use of a single platform to combine the technology ofultrahigh-throughput screening, next-generation sequencing, and genome mining,thus opening up unique opportunities for antibiotic discovery.
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ISSN	20758251
Journal	Acta Naturae
Volume Number	10
PubMed Central reference number	PMC6209406
Issue Number	3
PubMed reference number	30397523
Language	English
Publisher	A.I. Gordeyev
Publisher Date	2018-07-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of theCreative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original work isproperly cited. Copyright ® 2018 Park-media Ltd.
Subject Keyword	high-throughput screening antibiotic discovery antibiotic resistance microfluidics
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Molecular Medicine Biotechnology