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MicroRNA-140-5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β-catenin signaling pathway.
| Content Provider | Europe PMC |
|---|---|
| Author | Cha, Yinlian He, Ying Ouyang, Kaobin Xiong, Hailin Li, Jun Yuan, Xia |
| Copyright Year | 2018 |
| Abstract | MicroRNAs have been suggested as potential regulators in gastric cancer (GC) development through affecting the expression of their target genes. Previous studies have demonstrated that miR-140-5p is downregulated in GC. However, the underlying functional role of miR-140-5p in GC remains largely unknown. The present study revealed that miR-140-5p expression was significantly decreased in 60 GC tissues, compared with corresponding adjacent non-tumor tissues. A lower miR-140-5p expression was significantly associated with lymph node metastasis and an advanced Tumor-Node-Metastasis stage in patients with GC. Furthermore, patients with a lower miR-140-5p expression exhibited shorter disease-free survival and overall survival times. Gain- and loss-of-function assays revealed that increased miR-140-5p expression significantly inhibited GC cell proliferation and invasion ability, as well as the Wnt/β-catenin signaling pathway by decreasing WNT1 and β-catenin expression. However, decreasing miR-140-5p expression had the opposite effects. Bioinformatics methods and dual-luciferase reporter assays revealed that WNT1 was a direct target of miR-140-5p. miR-140-5p suppressed cell proliferation and invasion by regulating WNT1 expression. Therefore, the results of the present study demonstrated that miR-140-5p may serve as a potential prognostic marker and therapeutic target in patients with GC. |
| ISSN | 17921074 |
| Journal | Oncology Letters |
| Volume Number | 16 |
| PubMed Central reference number | PMC6202481 |
| Issue Number | 5 |
| PubMed reference number | 30405773 |
| e-ISSN | 17921082 |
| DOI | 10.3892/ol.2018.9480 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2018-09-21 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Cha et al. |
| Subject Keyword | gastric cancer microRNA-140-5p WNT1 cell proliferation cell invasion |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
