A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples.

Content Provider	Europe PMC
Author	Yang, Xingdong Sun, Zhongke Tian, Fengshou Jia, Guochao Yang, Jifei Hu, Xiaofei
Copyright Year	2018
Abstract	A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg−1, and the limit of detection value was 0.62 µg kg−1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.
Volume Number	5
PubMed Central reference number	PMC6124078
Issue Number	8
PubMed reference number	30225042
Journal	Royal Society Open Science [R Soc Open Sci]
e-ISSN	20545703
DOI	10.1098/rsos.180504
Language	English
Publisher	The Royal Society
Publisher Date	2018-08-29
Access Restriction	Open
Rights License	Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. © 2018 The Authors.
Subject Keyword	hexoestrol immunoassay colloidal gold lateral flow immunochromatographic strip test (LFIST) rapid test
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary