Effects of Oncostatin M on Invasion of Primary Trophoblasts under Normoxia and Hypoxia Conditions.

Content Provider	Europe PMC
Author	Wie, Jeong ha Ko, Hyun Sun Choi, Sae Kyung Park, In Yang Kim, Ahyoung Kim, Ho Shik Shin, Jong Chul
Copyright Year	2018
Abstract	PurposeTo investigate the effect of oncostatin M (OSM) on protein expression levels and enzymatic activities of matrix metalloprotainase (MMP)-2 and MMP-9 in primary trophoblasts and the invasiveness thereof under normoxia and hypoxia conditions.Materials and MethodsProtein expression levels and enzymatic activities of MMP-2 and MMP-9 in primary trophoblasts under normoxia and hypoxia conditions were examined by Western blot and zymography, respectively. Effects of exogenous OSM on the in vitro invasion activity of trophoblasts according to oxygen concentration were also determined. Signal transducer and activator of transcription 3 (STAT3) siRNA was used to determine whether STAT3 activation in primary trophoblasts was involved in the effect of OSM.ResultsOSM enhanced protein expression levels and enzymatic activities of MMP-2 and MMP-9 in term trophoblasts under hypoxia condition, compared to normoxia control (p<0.05). OSM-induced MMP-2 and MMP-9 enzymatic activities were significantly suppressed by STAT3 siRNA silencing under normoxia and hypoxia conditions (p<0.05). Hypoxia alone or OSM alone did not significantly increase the invasiveness of term trophoblasts. However, the invasion activity of term trophoblasts was significantly increased by OSM under hypoxia, compared to that without OSM treatment under normoxia.ConclusionOSM might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts.
ISSN	05135796
Volume Number	59
PubMed Central reference number	PMC6082983
Issue Number	7
PubMed reference number	30091322
Journal	Yonsei Medical Journal [Yonsei Med. J]
e-ISSN	19762437
DOI	10.3349/ymj.2018.59.7.879
Language	English
Publisher	Yonsei University College of Medicine
Publisher Date	2018-09-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © Copyright: Yonsei University College of Medicine 2018
Subject Keyword	Oncostatin M trophoblast signal transducer and activator of transcription 3 hypoxia
Content Type	Text
Resource Type	Article
Subject	Medicine